| Long name: |
Cy5, 5 Conjugated, RIPK1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy5, 5, 5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RIPK1 |
| Specificity: |
This is a highly specific antibody against RIPK1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
185-230/671 |
| Subcellular location: |
Cell membrane, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human RIPK1 |
| Gene ID number: |
8737 |
| Swiss Prot: |
Q13546 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death, Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, Polyubiquitinated protein binds to IKBKG/NEMO, RIPK1 and RIPK3 association, TRADD and TRAF2 are recruited to the receptor, Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, Upon activation of TNFR1 by the TNF-alpha family cytokines, a critical event for NF-kappa-B activation, activation of pathogen recognition receptors (PRRs), and DNA damage, and thereby activates the MAP3K5-JNK apoptotic cascade, forms a necrosis-inducing complex, in particular, the regulatory subunit of the IKK complex, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules, Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
675nm/694nm |
| Synonyms: |
RIP |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths |
| Gene target: |
RIPK1 Conjugated |
| Short name: |
Conjugated, RIPK1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
5, Cy5 |
| Alternative name: |
cyanine 5, 5 coupled, receptor (TNFRSF)-interacting serine-threonine kinase 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Plasma membranes, RIP and RIP1, RIPK1 and IDBG-57326 and ENSG00000137275 and 8737, RIPK1 and IDBG-636679 and ENSBTAG00000006378 and 504727, Ripk1 and IDBG-143710 and ENSMUSG00000021408 and 19766, this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005123 and death receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0007165 and signal transduction and biological process this GO :0007257 and activation of JUN kinase activity and biological process this GO :0010942 and positive regulation of cell death and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005123 : death receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005123 : death receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0031625 : ubiquitin protein ligase binding, this GO :0032403 : protein complex binding, this GO :0042802 : identical protein binding, this GO :0070513 : death domain binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0032403 : protein complex binding and also this GO :0042802 : identical protein binding and also this GO :0070513 : death domain binding, transferring phosphorus-containing groups and molecular function this GO :0031264 and death-inducing signaling complex and cellular component this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0032481 and positive regulation of type I interferon production and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0033209 and tumor necrosis factor-mediated signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0036289 and peptidyl-serine autophosphorylation and biological process this GO :0042327 and positive regulation of phosphorylation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043068 and positive regulation of programmed cell death and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043124 and negative regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043234 and protein complex and cellular component this GO :0043235 and receptor complex and cellular component this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0044257 and cellular protein catabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045651 and positive regulation of macrophage differentiation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051260 and protein homooli this GO merization and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051347 and positive regulation of transferase activity and biological process this GO :0060545 and positive regulation of necroptotic process and biological process this GO :0070231 and T cell apoptotic process and biological process this GO :0070266 and necroptotic process and biological process this GO :0070513 and death domain binding and molecular function this GO :0070926 and regulation of ATP:ADP antiporter activity and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097342 and ripoptosome and cellular component this GO :0097343 and ripoptosome assembly and biological process this GO :0097527 and necroptotic signaling pathway and biological process this GO :1901026 and ripoptosome assembly involved in necroptotic process and biological process this GO :1990000 and amyloid fibril formation and biological process this GO :2000377 and regulation of reactive oxygen species metabolic process and biological process this GO :2000379 and positive regulation of reactive oxygen species metabolic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process this GO :2001238 and positive regulation of extrinsic apoptotic signaling pathway and biological process this GO :2001239 and regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Identity: |
10019 |
| Gene: |
RIPK1 |
More about : RIPK1 |
| Long gene name: |
receptor interacting serine/threonine kinase 1 |
| Synonyms gene name: |
receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Synonyms name: |
receptor-interacting protein kinase 1 |
| Locus: |
6p25, 2 |
| Discovery year: |
1999-05-07 |
| GenBank acession: |
U25994 |
| Entrez gene record: |
8737 |
| Pubmed identfication: |
7538908 8612133 |
| RefSeq identity: |
NM_003804 |
| Classification: |
Ripoptosome |
| Havana BLAST/BLAT: |
OTTHUMG00000014134 |