| Long name: |
FITC Conjugated, Glycophorin B Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GYPB |
| Specificity: |
This is a highly specific antibody against GYPB |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
20-59/91 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Glycophorin B |
| Gene ID number: |
2994 |
| Swiss Prot: |
P06028 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
B and C are sialoglycoproteins of the human erythrocyte membrane, Glycophorin C transcript can generate two protein isoforms, Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte, Isoform 1 includes all 4 exons and encodes the full length 128 amino acid protein, Isoform 2 is missing exon 2 and encodes a 109 amino acid protein, Ss and Gerbich blood groups, The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual, The human Glycophorin A gene maps to chromosome 4q31, The human Glycophorin B gene maps to chromosome 4q31, The human Glycophorin C gene maps to chromosome 2q14, and encodes a 150 amino acid protein, contains seven exons which are 97% homologous to Glycophorin B, respectively, which bear the antigenic determinants for the MN, which specifies the Yus subtype of the Gerbich phenotype, 21, 21 and encodes a 91 amino acid protein, 3 and contains four exons, Glycophorins A |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CD235b, GPB, GPB, GYPB, GYPHe, Glycophorin-B, GpMiIII, HGpMiIII, HGpMiVI, HGpMiX, MNS, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, NY, NY, SS |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Glycophorin B Conjugated |
| Short name: |
FITC Conjugated, Glycophorin B Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Glycophorin B (Antibody to) |
| Alternative technique: |
antibodies |