| Long name: |
c-Kit Primary Polyclonal Antibody |
| Also known as: |
c-Kit PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
c-Kit |
| Specificity: |
This is a highly specific antibody against c-Kit |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
360-405/976 |
| Subcellular location: |
Extracellular |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human c-Kit |
| Gene ID number: |
3815 |
| Swiss Prot: |
P10721 |
| Tested Applications: |
IF(ICC), IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Activated KIT also transmits signals via GRB2 and activation of RAS, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1, CBL, CRK (isoform Crk-II), In response to KITLG/SCF binding, KIT can activate several signaling pathways, KIT signaling is modulated by protein phosphatases, LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, PLCG1, Phosphorylates PIK3R1, Promotes activation of STAT family members STAT1, Promotes phosphorylation of PIK3R1, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, SH2B2/APS and CBL, SRC and SHC1, STAT3, STAT3, STAT5A and STAT5B, STAT5A and STAT5B, and by rapid internalization and degradation of the receptor, and in melanogenesis, and of the transcription factors STAT1, gametogenesis, hematopoiesis, mast cell development, migration and function, stem cell maintenance, the regulatory subunit of phosphatidylinositol 3-kinase, 4, 5-trisphosphate, Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
C-Kit, CD117, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
c-Kit |
| Short name: |
c-Kit Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
c-reagent (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8631 |
| Gene: |
PBT |
More about : PBT |
| Long gene name: |
KIT proto-oncogene receptor tyrosine kinase |
| Synonyms gene name: |
piebald trait v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Synonyms: |
CD117 SCFR C-Kit KIT |
| Locus: |
4q12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
S67773 |
| Entrez gene record: |
3815 |
| Pubmed identfication: |
9027509 |
| RefSeq identity: |
NM_000222 |
| Classification: |
CD molecules Immunoglobulin like domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000128713 |
| Locus Specific Databases: |
LRG_307 |