| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
c-Kit |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the c-Kit antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This c-Kit antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
3815 |
| Purity: |
Antigen affinity |
| Description: |
Determination of the c-Kit effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells, It is expressed on the surface of germ cells up to the pachytene stage, Signaling from the receptor, also known as CD117, c-Kit was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit, is a protein that in humans is encoded by the KIT gene, tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro, SCFR (Mast/stem cell growth factor receptor) |
| Immunogen: |
Human protein shares 66% amino acid sequence identity with mouse protein, Human partial recombinant protein (AA 26-285) was used as the immunogen for this c-Kit antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the c-Kit antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
c-Kit |
| Short name: |
c-Kit Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
c-reagent (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8631 |
| Gene: |
PBT |
More about : PBT |
| Long gene name: |
KIT proto-oncogene receptor tyrosine kinase |
| Synonyms gene name: |
piebald trait v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Synonyms: |
CD117 SCFR C-Kit KIT |
| Locus: |
4q12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
S67773 |
| Entrez gene record: |
3815 |
| Pubmed identfication: |
9027509 |
| RefSeq identity: |
NM_000222 |
| Classification: |
CD molecules Immunoglobulin like domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000128713 |
| Locus Specific Databases: |
LRG_307 |