| Long name: |
FITC Conjugated, Histone H1 (Lys52) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Acetyl-Histone H1 Lys52 |
| Specificity: |
H1, H1, H1, This antibody will react with members of the Histone H1 family that have an acetylated lysine positioned in the motif AS(Ac-K)ER, This include H1, and H1, 1, 2, 3, 4, 5, This is a highly specific antibody against Acetyl-Histone H1 Lys52 |
| Modification: |
Acetylation |
| Modification site: |
Lys52 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-60/219 |
| Subcellular location: |
Nucleus |
| Source: |
4 around acetylation site of Lys52, KLH conjugated synthetic acetylpeptide derived from human Histone H1 |
| Gene ID number: |
3008 |
| Swiss Prot: |
P10412 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts also as a regulator of individual gene transcription through chromatin remodeling, Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers, nucleosome spacing and DNA methylation (By similarity), Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
H1, H1F4, H1s-4, HIST1H1E, Histone H1, Histone H1b, Histone H1s-4, dJ221C16, 4, 4, 5, H1E |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Histone H1 (Lys52) Conjugated |
| Short name: |
FITC Conjugated, Histone H1 (Lys52) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Histone H1 (Lys52) (Antibody to) |
| Alternative technique: |
antibodies |