| Long name: |
FITC Conjugated, Collagen IV Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Collagen IV |
| Specificity: |
This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain, This is a highly specific antibody against Collagen IV |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1430-1480/1669 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain |
| Gene ID number: |
12826 |
| Swiss Prot: |
P02463 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Arresten, Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation, Ligand for alpha1/beta1 integrin (By similarity), Specifically inhibits endothelial cell proliferation, The C-terminal half is found to possess the anti-angiogenic activity, comprising the C-terminal NC1 domain, forming a 'chicken-wire' meshwork together with laminins, inhibits angiogenesis and tumor formation, migration and tube formation, proteoglycans and entactin/nidogen, Type IV collagen is the major structural component of glomerular basement membranes (GBM) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Col4a-1, Col4a1, Collagen alpha-1(IV) chain, Del(8)44H, Raw, Svc, Bru |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Collagen IV Conjugated |
| Short name: |
FITC Conjugated, Collagen IV Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Collagen IV (Antibody to) |
| Alternative technique: |
antibodies |