| Long name: |
Biotin Conjugated, Livin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Livin |
| Specificity: |
This is a highly specific antibody against Livin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
100-150/298 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human Livin |
| Gene ID number: |
79444 |
| Swiss Prot: |
Q96CA5 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
As it is a weak caspase inhibitor, CASP7 and CASP9, In vitro, Isoform 1 blocks staurosporine-induced apoptosis, Isoform 2 blocks etoposide-induced apoptosis, Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing, Its anti-apoptotic activity is mediated through the inhibition of CASP3, May contribute to caspase inhibition, Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, Suppression of apoptosis is mediated by activation of MAPK8/JNK1, This activation depends on TAB1 and NR2C2/TAK1, and cell cycle control, and possibly also of MAPK9/JNK2, as well as by its E3 ubiquitin-protein ligase activity, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions, cell proliferation, etoposide or staurosporine, inhibits CASP3 and proteolytic activation of pro-CASP9, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival, Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, LIVIN, ML-IAP, MLIAP, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RNF50, UNQ5800/PRO19607/PRO21344, KIAP |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Livin Conjugated |
| Short name: |
Conjugated, Livin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Livin (Antibody to) |
| Alternative technique: |
antibodies |