| Long name: |
Biotin Conjugated, c-Kit Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
c-Kit |
| Specificity: |
This is a highly specific antibody against c-Kit |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
928-964/988 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human CD117/c-kit |
| Gene ID number: |
3815 |
| Swiss Prot: |
P10721 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Dog, Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activated KIT also transmits signals via GRB2 and activation of RAS, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1, CBL, CRK (isoform Crk-II), In response to KITLG/SCF binding, KIT can activate several signaling pathways, KIT signaling is modulated by protein phosphatases, LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, PLCG1, Phosphorylates PIK3R1, Promotes activation of STAT family members STAT1, Promotes phosphorylation of PIK3R1, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, SH2B2/APS and CBL, SRC and SHC1, STAT3, STAT3, STAT5A and STAT5B, STAT5A and STAT5B, and by rapid internalization and degradation of the receptor, and in melanogenesis, and of the transcription factors STAT1, gametogenesis, hematopoiesis, mast cell development, migration and function, stem cell maintenance, the regulatory subunit of phosphatidylinositol 3-kinase, 4, 5-trisphosphate, Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
C-Kit, CD117, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
c-Kit Conjugated |
| Short name: |
Conjugated, c-Kit Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, c-reagent (Antibody to) |
| Alternative technique: |
antibodies |