| Long name: |
Biotin Conjugated, HMGB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HMGB1 |
| Specificity: |
This is a highly specific antibody against HMGB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
60-110/215 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human HMGB1 |
| Gene ID number: |
3146 |
| Swiss Prot: |
P09429 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS), Binds preferentially single-stranded DNA, Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity), Involved in V(D)J recombination by acting as a cofactor of the RAG complex, DNA binding proteins that associates with chromatin and has the ability to bend DNA |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
HMG3 SBP-1 DKFZp686A04236 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HMGB1 Conjugated |
| Short name: |
Conjugated, HMGB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, high mobility family box 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA binding, DNA ligation and biological process this GO :0006309 and apoptotic DNA fragmentation and biological process this GO :0006310 and DNA recombination and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006915 and apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008301 and DNA binding, HMG1 and HMG3 and SBP-1, HMGB1 and IDBG-21100 and ENSG00000189403 and 3146, HMGB1 and IDBG-630533 and ENSBTAG00000018103 and 282691, Hmgb1 and IDBG-209311 and ENSMUSG00000066551 and 100862258, bending, bending and also this GO :0042056 : chemoattractant activity and also this GO :0044822 : poly(A) RNA binding and also this GO :0050786 : RAGE receptor binding and also this GO :0070491 : repressing transcription factor binding, bending and molecular function this GO :0009986 and cell surface and cellular component this GO :0017053 and transcriptional repressor complex and cellular component this GO :0017055 and negative regulation of RNA polymerase II transcriptional preinitiation complex assembly and biological process this GO :0031175 and neuron projection development and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043280 and positive regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043388 and positive regulation of DNA binding and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050786 and RAGE receptor binding and molecular function this GO :0050918 and positive chemotaxis and biological process this GO :0051103 and DNA ligation involved in DNA repair and biological process this GO :0070491 and repressing transcription factor binding and molecular function this GO :2000426 and negative regulation of apoptotic cell clearance and biological process, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0001773 and myeloid dendritic cell activation and biological process this GO :0002407 and dendritic cell chemotaxis and biological process this GO :0002437 and inflammatory response to antigenic stimulus and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006265 and DNA topological change and biological process this GO :0006288 and base-excision repair, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003684 : damaged DNA binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0008301 : DNA binding, this GO :0003684 : damaged DNA binding, this GO :0003690 : double-stranded DNA binding, this GO :0003697 : single-stranded DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005125 : cytokine activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0008301 : DNA binding, this GO :0042056 : chemoattractant activity, this GO :0044822 : poly(A) RNA binding, this GO :0050786 : RAGE receptor binding, this GO :0070491 : repressing transcription factor binding, 15289, 619937, 102642363, high mobility group box 1 |
| Identity: |
4983 |
| Gene: |
HMGB1 |
More about : HMGB1 |
| Long gene name: |
high mobility group box 1 |
| Synonyms gene: |
HMG1 |
| Synonyms gene name: |
high-mobility group (nonhistone chromosomal) protein 1 high-mobility group box 1 |
| Synonyms name: |
high mobility group box 1 Sulfoglucuronyl carbohydrate binding protein Amphoterin high mobility group protein 1 |
| Locus: |
13q12, 3 |
| Discovery year: |
1993-12-13 |
| GenBank acession: |
D63874 |
| Entrez gene record: |
3146 |
| Pubmed identfication: |
8661151 11279268 |
| RefSeq identity: |
NM_002128 |
| Classification: |
Canonical high mobility group |
| Havana BLAST/BLAT: |
OTTHUMG00000016670 |