| Long name: |
FITC Conjugated, Integrin alpha 4/CD49d Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Integrin alpha 4/CD49d |
| Specificity: |
This is a highly specific antibody against Integrin alpha 4/CD49d |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
584-609/1032 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Integrin alpha 4 |
| Gene ID number: |
3676 |
| Swiss Prot: |
P13612 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rabbit, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1, Integrin alpha-4/beta-7 is also a receptor for MADCAM1, It may also participate in cytolytic T-cell interactions with target cells, It recognizes the sequence L-D-T in MADCAM1, On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines, They are also receptors for VCAM1, They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin, Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CD49 antigen-like family member D, CD49D, ITGA4, Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha, IA4 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Integrin alpha 4/CD49d Conjugated |
| Short name: |
FITC Conjugated, Integrin alpha 4/CD49d Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Integrin a 4/CD49d (Antibody to) |
| Alternative technique: |
antibodies |