| Long name: |
ALEXA FLUOR 488 Conjugated, CD31 Polyclonal Antibody |
| Also known as: |
Anti-CD31 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CD31 |
| Specificity: |
This is a highly specific antibody against CD31 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
621-650/738 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from mouse CD31 |
| Gene ID Number: |
5175 |
| Swiss Prot: |
P16284 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Dog, Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions, During apoptosis, Isoform Delta15 is unable to protect against apoptosis, Modulates BDKRB2 activation, Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC), The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, Tyr-69 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore, thus triggering the process of engulfment), Induces susceptibility to atherosclerosis (By similarity) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
CD31 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the CD31 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
CD31 |
| Short name: |
CD31 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, CD31 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8823 |
| Gene: |
PECAM1 |
More about : PECAM1 |
| Long gene name: |
platelet and endothelial cell adhesion molecule 1 |
| Synonyms gene name: |
platelet/endothelial cell adhesion molecule 1 |
| Synonyms name: |
CD31 antigen |
| Locus: |
17q23, 3 |
| Discovery year: |
1995-11-29 |
| GenBank acession: |
L34631 M37780 |
| Entrez gene record: |
5175 |
| Pubmed identfication: |
8661055 1690453 |
| RefSeq identity: |
NM_000442 |
| Classification: |
CD molecules Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000175829 |