| Long name: |
GAPDH Primary Polyclonal Antibody |
| Also known as: |
GAPDH PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
GAPDH |
| Specificity: |
This is a highly specific antibody against GAPDH |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
90-140/333 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from mouse GAPDH |
| Gene ID number: |
14433 |
| Swiss Prot: |
P16858 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Also participates in nuclear events including transcription, Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes, DNA replication and apoptosis, Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules, Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate, HDAC2 and PRKDC, Modulates the organization and assembly of the cytoskeleton, Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, RNA transport, Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation, respectively, thereby playing a role in glycolysis and nuclear functions, Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, Gapd |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GAPDH |
| Short name: |
GAPDH Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GAPDH (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4141 |
| Gene: |
GAPDH |
More about : GAPDH |
| Long gene name: |
glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms gene: |
GAPD |
| Locus: |
12p13, 31 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AF261085 |
| Entrez gene record: |
2597 |
| Pubmed identfication: |
3170585 |
| RefSeq identity: |
NM_002046 |
| Havana BLAST/BLAT: |
OTTHUMG00000137379 |