| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GAPDH |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This GAPDH antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P04406 |
| Purity: |
Antigen affinity |
| Description: |
Due to its ubiquitous presence in mammalian cells, GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, GAPDH is often used a loading control protein in western blot, The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus, The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions, The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), typically transfected vs mock or non transfected cell lysate, Glyceraldehyde 3-phosphate dehydrogenase is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules |
| Immunogen: |
An amino acid sequence from the C-terminus of human GAPDH (YSNRVVDLMAHMASKE) was used as the immunogen for this GAPDH antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GAPDH antibody can be stored for up to one month at 4oC, After reconstitution |
| Localization: |
Nuclear and cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GAPDH |
| Short name: |
GAPDH Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GAPDH (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4141 |
| Gene: |
GAPDH |
More about : GAPDH |
| Long gene name: |
glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms gene: |
GAPD |
| Locus: |
12p13, 31 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AF261085 |
| Entrez gene record: |
2597 |
| Pubmed identfication: |
3170585 |
| RefSeq identity: |
NM_002046 |
| Havana BLAST/BLAT: |
OTTHUMG00000137379 |