| Long name: |
FITC Conjugated, Streptavidin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Streptavidin |
| Specificity: |
This is a highly specific antibody against Streptavidin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
Recombinant Streptavidin |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Others |
| Crossreactive species details: |
Streptavidin |
| Antigen background: |
Guanidinium chloride will dissociate avidin and streptavidin into subunits, In contrast to avidin, Streptavidin is a tetrameric protein, Streptavidin products use a recombinant form of streptavidin having a mass of 53, but streptavidin is more resistant to dissociation, streptavidin has no carbohydrate and has a mildly acidic pI of 5, with each subunit binding one molecule of biotin with affinity similar to that of avidin, 000 daltons and a near-neutral pI, Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SA V1, SA V2, SAV1_STRVL, Streptavidin V1, Streptavidin V2, SA protein |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Streptavidin Conjugated |
| Short name: |
FITC Conjugated, Streptavidin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, biotinilated-binding streptavidin (Antibody to) |
| Alternative technique: |
antibodies |