| Long name: |
FITC Conjugated, Histone H3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Histone H3 |
| Specificity: |
This is a highly specific antibody against Histone H3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
90-136/136 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human Histone H3 |
| Gene ID number: |
3020 |
| Swiss Prot: |
P84243 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Pig, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis, DNA accessibility is regulated via a complex set of post-translational modifications of histones, DNA repair, DNA replication and chromosomal stability, Deposited at sites of nucleosomal displacement throughout transcribed genes, Histones thereby play a central role in transcription regulation, Nucleosomes wrap and compact DNA into chromatin, also called histone code, and nucleosome remodeling, limiting DNA accessibility to the cellular machineries which require DNA as a template, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin, Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
H3, H3F3A, Histone H3, PP781, 3, 3A, H3F3 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Histone H3 Conjugated |
| Short name: |
FITC Conjugated, Histone H3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Histone H3 (Antibody to) |
| Alternative technique: |
antibodies |