| Category: |
Conjugated Secondary Antibody |
| Conjugation: |
Biotinylated |
| Host Organism: |
Donkey |
| Target antigen: |
Human IgG |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal antibody |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug/ul |
| Tested applications: |
ELISA, IHC-F, IHC-P, WB |
| Recommended dilutions: |
ELISA(1:500-2000), IHC-F(1:500-2000), IHC-P(1:500-2000), WB(1:500-2000) |
| Cross reactivity: |
Human |
| Background of the target antigen: |
IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response, The IgG molecule has two separate functions, The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants, is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen, Immunoglobulin G (IgG) |
| Purification method: |
This antibody has been purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to one year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, 6, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°, humans , ssp |
| Latin name: |
Equus africanus asinus |
| About: |
2 light chains, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Gene target: |
(H+L) Conjugated |
| Short name: |
Anti- IgG Antibody (H+L) Conjugated |
| Technique: |
IgGs, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Host: |
Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs, For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human, We provide also anti-donkey as a species antibodies and ELISA kits, Donkeys or Equus africanus asinus are often used as host species to raise antibodies |
| Isotype: |
IgG |
| Label: |
Biotin |
| Species: |
Donkeys, Humans, Human |
| Alternative name: |
biotinilated coupled, sapiens Immunoglobulin G (Antibody to) (heavy and light chain), Donkey antibody to-H |
| Alternative technique: |
antibodies |