| Long name: |
FITC Conjugated, VEGF Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
VEGF |
| Specificity: |
This is a highly specific antibody against VEGF |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
27-77/232 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from human VEGF |
| Gene ID number: |
7422 |
| Swiss Prot: |
P15692 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, Induces endothelial cell proliferation, Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145, does not activate angiogenesis and inhibits tumor growth, heparan sulfate and heparin, inhibits apoptosis and induces permeabilization of blood vessels, promotes cell migration, vasculogenesis and endothelial cell growth, Growth factor active in angiogenesis |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
VEGF-A VPF |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
VEGF Conjugated |
| Short name: |
FITC Conjugated, VEGF Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, VEGF (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12680 |
| Gene: |
VEGFA |
More about : VEGFA |
| Long gene name: |
vascular endothelial growth factor A |
| Synonyms gene: |
VEGF |
| Synonyms gene name: |
vascular endothelial growth factor |
| Locus: |
6p21, 1 |
| Discovery year: |
1994-01-10 |
| GenBank acession: |
AB021221 |
| Entrez gene record: |
7422 |
| Pubmed identfication: |
8786112 |
| RefSeq identity: |
NM_001025366 |
| Classification: |
VEGF family |
| Havana BLAST/BLAT: |
OTTHUMG00000014745 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |