| Long name: |
FITC Conjugated, KLH Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Mouse (Mus musculus) |
| Target Antigen: |
KLH |
| Specificity: |
This is a highly specific antibody against KLH |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH protein |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Others |
| Crossreactive species details: |
Keyhole limpet hemocyanin (KLH) |
| Antigen background: |
Aggregates remain immunogenic, Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2), Haptens are substances with a low molecular weight such as peptides, In addition, It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata, Its large size and numerous epitopes generate a substantial immune response, KLH is an effective carrier protein for several reasons, The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein, The large and highly glycosylated KLH protein cannot be reproduced synthetically, When oxygen is bound to hemocyanin, a gastropod, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific , and abundance of lysine residues for coupling haptens, because KLH is derived from the limpet, biotechnology and therapeutic applications, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350, it is phylogenetically distant from mammalian proteins, opalescent blue color, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production, the molecule takes on a distinctive transparent, thus reducing false positives in immunologically based research techniques in mammalian model organisms, 000, 000, 000 and 390, 000 in aggregates with molecular weights of 4, 000-13, 500, KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate, Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, Keyhole limpet hemocyanin is an extremely large, [2] KLH is the most widely employed carrier proteins for this purpose |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Keyhole limpet hemocyanin KLH |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KLH Conjugated |
| Short name: |
FITC Conjugated, KLH Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, KLH (Antibody to) |
| Alternative technique: |
antibodies |