| We send this reagant on: |
Blue Ice |
| The temperatureerature for storage is: |
Store all other components at 4º, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20º, C, C and avoid multiple freeze/thaw cycles, Upon receiving |
| Technical file: |
more kit, pdf |
| SDS or safty data sheet: |
AKR-172) GHS, details (AKR-171, pdf |
| Type of reagent: |
Cell Signaling and Protein Biology |
| Related techniques: |
Nuclear/Cytosolic Fractionation test kits, Protein Analysis Tools, Protein Isolation and Extraction, Cell Signaling and Protein Biology |
| Use of the reagent: |
Nuclear/Cytosolic Fractionation test kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells, RNA splicing, The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, The procedure has been optimized to provide extraction, and Western blotting, enzyme activity assays, gel shift assays (EMSA), in less than 2 hours, reporter assays, with high protein recovery and low cross-contamination, , , Cell Biolabs&rsquo |
| Cellular use: |
Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells |
| Protocol information: |
Extraction in less than 2 hours |
| Reagent properties: |
Each prep can accommodate , up to 5 x 106cells |
| Conjugation and form: |
host specific |
| Species compatible to: |
human cells and cell lines and other cells |
| Use of this test kit or product: |
Molecular biology |
| Antigen: |
the sequence of the protein |
| Buffer: |
19, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
kiloDalton |
| This reagent is purified: |
98 |
| Gene target: |
/Cytosolic Fractionation test kit |
| Short name: |
Nuclear/Cytosolic Fractionation test kit |
| Alternative name: |
Nuclear/Cytosolic Fractionation test reagent |
| Alternative technique: |
tests |