| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human B Lymphocyte Kinase is produced by our E, coli expression system and the target gene encoding Gly2-Pro505 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
58, 7 kD |
| UniProt number: |
P51451 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry Ice/ice packs |
| Formulation: |
1 mM DTT, 500 mM sodium chloride, pH 7, 2 um filtered solution of 20 mM Tris, 4, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
GLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQPLEHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
BLK (C-6His), Tyrosine-Protein Kinase Blk |
| Short name: |
BLK (C-6His), Recombinant Tyrosine-Protein Kinase Blk |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
B lymphoid tyrosine kinase (C-6His), sapiens Tyrosine-Protein phosphorylation catalyst B lymphoid tyrosine kinase, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
BLK and IDBG-629427 and ENSBTAG00000005049 and 532587, BLK and IDBG-7345 and ENSG00000136573 and 640, Blk and IDBG-172522 and ENSMUSG00000014453 and 12143, MODY11, Plasma membranes, this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0032024 and positive regulation of insulin secretion and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0050853 and B cell receptor signaling pathway and biological process, B lymphoid tyrosine kinase |
| Identity: |
1057 |
| Gene: |
BLK |
More about : BLK |
| Long gene name: |
BLK proto-oncogene, Src family tyrosine kinase |
| Synonyms gene name: |
B lymphoid tyrosine kinase |
| Synonyms: |
MGC10442 |
| Locus: |
8p23, 1 |
| Discovery year: |
1995-05-17 |
| GenBank acession: |
BC004473 |
| Entrez gene record: |
640 |
| Pubmed identfication: |
7845672 |
| Classification: |
SH2 domain containing Src family tyrosine kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000090729 |