| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human NCK1 is produced by our E, coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus |
| Molecular Weight: |
45 kD |
| UniProt number: |
P16333 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
50 mM sodium chloride, pH 8, 2 um filtered solution of 20 mM Tris, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MGSSHHHHHHSSGLVPRGSHMAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
Cytoplasmic Protein NCK1 (N-6His) |
| Short name: |
Recombinant Cytoplasmic Protein NCK1 (N-6His) |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
sapiens Cytoplasmic Protein NCK adaptor protein 1 (N-6His), recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
NCK and nck-1 and NCKalpha, NCK1 and IDBG-58017 and ENSG00000158092 and 4690, NCK1 and IDBG-638662 and ENSBTAG00000018427 and 536154, Nck1 and IDBG-193200 and ENSMUSG00000032475 and 17973, nuclei, receptor tyrosine kinase binding, this GO :0004860 and protein kinase inhibitor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006417 and regulation of translation and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0007015 and actin filament organization and biological process this GO :0007172 and signal complex assembly and biological process this GO :0007411 and axon guidance and biological process this GO :0008093 and cytoskeletal adaptor activity and molecular function this GO :0012506 and vesicle membrane and cellular component this GO :0016477 and cell migration and biological process this GO :0019887 and protein kinase regulator activity and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0030032 and lamellipodium assembly and biological process this GO :0030159 and receptor signaling complex scaffold activity and molecular function this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0030971 and receptor tyrosine kinase binding and molecular function this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042110 and T cell activation and biological process this GO :0045087 and innate immune response and biological process this GO :0045859 and regulation of protein kinase activity and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0051707 and response to other organism and biological process this GO :0060548 and negative regulation of cell death and biological process, this GO :0004860 : protein kinase inhibitor activity, this GO :0004860 : protein kinase inhibitor activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0008093 : cytoskeletal adaptor activity and also this GO :0019887 : protein kinase regulator activity and also this GO :0019904 : protein domain specific binding and also this GO :0030159 : receptor signaling complex scaffold activity and also this GO :0030971 : receptor tyrosine kinase binding, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0008093 : cytoskeletal adaptor activity, this GO :0019887 : protein kinase regulator activity, this GO :0019904 : protein domain specific binding, this GO :0030159 : receptor signaling complex scaffold activity, this GO :0030971 : receptor tyrosine kinase binding, NCK adaptor protein 1 |
| Identity: |
7664 |
| Gene: |
NCK1 |
More about : NCK1 |
| Long gene name: |
NCK adaptor protein 1 |
| Synonyms gene: |
NCK |
| Synonyms: |
NCKalpha |
| Locus: |
3q22, 3 |
| Discovery year: |
1998-11-04 |
| GenBank acession: |
X17576 |
| Entrez gene record: |
4690 |
| Pubmed identfication: |
7806213 9737977 |
| RefSeq identity: |
NM_006153 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000159781 |