| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Semaphorin 4D is produced by our Mammalian expression system and the target gene encoding Met22-Arg734 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
2 kD, 80 |
| UniProt number: |
Q92854 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
pH 7, 150 mM sodium chloride, 2 um filtered solution of 20 mM PB, 4, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNDVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
CD100 (C-6His), SEMA4D, Semaphorin-4D |
| Short name: |
CD100 (C-6His), SEMA4D, Recombinant Semaphorin-4D |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
(semaphorin) 4D, CD100 (C-6His), immunoglobulin domain (Ig), sapiens Semaphorin-4D, sema domain, transmembrane domain (TM) and short cytoplasmic domain, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
(semaphorin) 4D, C9orf164 and CD100 and coll-4 and M-sema-G and SEMAJ, Extracellular, SEMA4D and IDBG-637043 and ENSBTAG00000001941 and 785942, SEMA4D and IDBG-75424 and ENSG00000187764 and 10507, Sema4d and IDBG-152500 and ENSMUSG00000021451 and 20354, immunoglobulin domain (Ig), semaphorin receptor binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0010693 and negative regulation of alkaline phosphatase activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030335 and positive regulation of cell migration and biological process this GO :0031344 and regulation of cell projection organization and biological process this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043931 and ossification involved in bone maturation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0048672 and positive regulation of collateral sprouting and biological process this GO :0048814 and regulation of dendrite morphogenesis and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050732 and negative regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0070486 and leukocyte aggregation and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process this GO :1900220 and semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0030215 : semaphorin receptor binding, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0030215 : semaphorin receptor binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10732 |
| Gene: |
SEMA4D |
More about : SEMA4D |
| Long gene name: |
semaphorin 4D |
| Synonyms gene: |
SEMAJ C9orf164 |
| Synonyms gene name: |
(semaphorin) 4D , chromosome 9 open reading frame 164 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
CD100 coll-4 FLJ39737 |
| Synonyms name: |
M-sema G |
| Locus: |
9q22, 2 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
U60800 |
| Entrez gene record: |
10507 |
| Pubmed identfication: |
8876214 8969198 |
| RefSeq identity: |
NM_006378 |
| Classification: |
CD molecules Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020185 |