| Antigen detectable with this ELISA kit: |
Semaphorin 4D (SEMA4D) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
57ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D), The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 4D (SEMA4D), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 4D (SEMA4D) |
| Short name: |
Semaphorin 4D (SEMA4D) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
(semaphorin) 4D) Enzyme-linked immunosorbent assay reagent, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, Semaphorin 4D (sema domain |
| Alternative technique: |
elisas |
| Alternative to gene target: |
(semaphorin) 4D, C9orf164 and CD100 and coll-4 and M-sema-G and SEMAJ, Extracellular, SEMA4D and IDBG-637043 and ENSBTAG00000001941 and 785942, SEMA4D and IDBG-75424 and ENSG00000187764 and 10507, Sema4d and IDBG-152500 and ENSMUSG00000021451 and 20354, immunoglobulin domain (Ig), semaphorin receptor binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0010693 and negative regulation of alkaline phosphatase activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030335 and positive regulation of cell migration and biological process this GO :0031344 and regulation of cell projection organization and biological process this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043931 and ossification involved in bone maturation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0048672 and positive regulation of collateral sprouting and biological process this GO :0048814 and regulation of dendrite morphogenesis and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050732 and negative regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0070486 and leukocyte aggregation and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process this GO :1900220 and semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0030215 : semaphorin receptor binding, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0030215 : semaphorin receptor binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10732 |
| Gene: |
SEMA4D |
More about : SEMA4D |
| Long gene name: |
semaphorin 4D |
| Synonyms gene: |
SEMAJ C9orf164 |
| Synonyms gene name: |
(semaphorin) 4D , chromosome 9 open reading frame 164 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
CD100 coll-4 FLJ39737 |
| Synonyms name: |
M-sema G |
| Locus: |
9q22, 2 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
U60800 |
| Entrez gene record: |
10507 |
| Pubmed identfication: |
8876214 8969198 |
| RefSeq identity: |
NM_006378 |
| Classification: |
CD molecules Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020185 |