| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant HumanGM-CSF is produced by our Mammalian expression system and the target gene encoding Ala18-Glu144 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
15, 5 kD |
| UniProt number: |
P04141 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
pH 7, 150 mM sodium chloride, 2 um filtered solution of 20 mM PB, 4, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQEVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Gene: |
CSF2 |
More about : CSF2 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
CSF2 (C-6His, Cells), GM-CSF |
| Short name: |
CSF2 (C-6His, Cells), Recombinant GM-CSF |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
H, colony stimulating factor 2 (granulocyte-macrophage) (C-6His, sapiens Cells), sapiens GM-CSF, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
CSF2 and IDBG-41275 and ENSG00000164400 and 1437, CSF2 and IDBG-644701 and ENSBTAG00000001570 and 281095, Csf2 and IDBG-172875 and ENSMUSG00000018916 and 12981, Extracellular, GMCSF, growth factor activity, this GO :0001892 and embryonic placenta development and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005129 and granulocyte macrophage colony-stimulating factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0032747 and positive regulation of interleukin-23 production and biological process this GO :0042045 and epithelial fluid transport and biological process this GO :0042116 and macrophage activation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0043011 and myeloid dendritic cell differentiation and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045918 and negative regulation of cytolysis and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071803 and positive regulation of podosome assembly and biological process this GO :0097028 and dendritic cell differentiation and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005129 : granulocyte macrophage colony-stimulating factor receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005129 : granulocyte macrophage colony-stimulating factor receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, colony stimulating factor 2 (granulocyte-macrophage) |
| Identity: |
2434 |
| Long gene name: |
colony stimulating factor 2 |
| Synonyms gene name: |
colony stimulating factor 2 (granulocyte-macrophage) |
| Synonyms: |
GM-CSF GMCSF |
| Synonyms name: |
sargramostim molgramostin granulocyte-macrophage colony stimulating factor |
| Locus: |
5q31, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M11734 |
| Entrez gene record: |
1437 |
| Pubmed identfication: |
3898082 2999978 |
| RefSeq identity: |
NM_000758 |
| Havana BLAST/BLAT: |
OTTHUMG00000059637 |