| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Kininogen-1 is produced by our Mammalian expression system and the target gene encoding Gln19-Ser427 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
46, 89 kD |
| UniProt number: |
P01042 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
150 mM sodium chloride, pH 4, 2 um filtered solution of 20 mM Hac-NaAC, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Avoid mixing using vortex or pipettes, Dissolve the protein in ddH2O, In order to retain the quality and the affinity of the product unchanged, It is not recommended to dilute the product to less than 100 &mu, avoid cycles of freezing and thawing, please, The vials should be centrifuged before it is opened, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVSLDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
KNG1 (C-6His), Kininogen-1 |
| Short name: |
KNG1 (C-6His), Recombinant Kininogen-1 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
kininogen 1 (C-6His), sapiens Kininogen-1, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
BDK and BK and KNG, Extracellular, KNG1 and IDBG-634652 and ENSBTAG00000005122 and 280833, KNG1 and IDBG-69059 and ENSG00000113889 and 3827, intrinsic pathway and biological process this GO :0008201 and heparin binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0030168 and platelet activation and biological process this GO :0030195 and negative regulation of blood coagulation and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0035810 and positive regulation of urine volume and biological process this GO :0035815 and positive regulation of renal sodium excretion and biological process this GO :0042311 and vasodilation and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072562 and blood microparticle and cellular component, this GO :0002576 and platelet degranulation and biological process this GO :0004869 and cysteine-type endopeptidase inhibitor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006939 and smooth muscle contraction and biological process this GO :0006954 and inflammatory response and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0007596 and blood coagulation and biological process this GO :0007597 and blood coagulation, this GO :0004869 : cysteine-type endopeptidase inhibitor activity, this GO :0004869 : cysteine-type endopeptidase inhibitor activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0008201 : heparin binding and also this GO :0008270 : zinc ion binding, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0008201 : heparin binding, this GO :0008270 : zinc ion binding, zinc ion binding, kininogen 1 |
| Identity: |
6383 |
| Gene: |
KNG1 |
More about : KNG1 |
| Long gene name: |
kininogen 1 |
| Synonyms gene: |
KNG BDK |
| Synonyms gene name: |
kininogen |
| Synonyms: |
BK |
| Synonyms name: |
alpha-2-thiol proteinase inhibitor bradykinin |
| Locus: |
3q27, 3 |
| Discovery year: |
1990-07-26 |
| Entrez gene record: |
3827 |
| Pubmed identfication: |
1733668 |
| RefSeq identity: |
NM_001102416 |
| Classification: |
Endogenous ligands Cystatins, type 3 |
| Havana BLAST/BLAT: |
OTTHUMG00000150348 |