| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Tet1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:2000 |
| Notes: |
Titration of the Tet1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Tet1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q3URK3 |
| Purity: |
Antigen affinity |
| Description: |
5fC and 5caC probably constitutes the first step in cytosine demethylation, Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters, Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), Conversion of 5mC into 5hmC, In addition to its role in DNA demethylation, Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification, Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation, Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes, [UniProt], and conversion of 5fC to 5-carboxylcytosine (5caC), plays a more general role in chromatin regulation, thereby promoting histone H2B GlcNAcylation by OGT, Tet1 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation |
| Immunogen: |
This mouse Tet1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1845-1879 amino acids from the C-terminal region of mouse Tet1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Tet1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Tet1 |
| Short name: |
Tet1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Tet1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
29484 |
| Gene: |
TET1 |
More about : TET1 |
| Long gene name: |
tet methylcytosine dioxygenase 1 |
| Synonyms gene: |
CXXC6 |
| Synonyms gene name: |
CXXC zinc finger 6 tet oncogene 1 |
| Synonyms: |
LCX KIAA1676 bA119F7, 1 |
| Synonyms name: |
leukemia-associated protein with a CXXC domain ten-eleven translocation-1 |
| Locus: |
10q21, 3 |
| Discovery year: |
2004-02-18 |
| GenBank acession: |
AF430147 |
| Entrez gene record: |
80312 |
| Pubmed identfication: |
12124344 12646957 |
| RefSeq identity: |
NM_030625 |
| Classification: |
Zinc fingers CXXC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000018359 |