| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Melk |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the Melk antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Melk antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q61846 |
| Purity: |
Antigen affinity |
| Description: |
Acts as a regulator of cell cycle, Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, CDC25B, Has a broad substrate specificity, MAP3K5/ASK1 and ZNF622, May also play a role in primitive hematopoiesis, Phosphorylates and inhibits BCL2L14, Plays a key role in cell proliferation, Required for proliferation of embryonic and postnatal multipotent neural progenitors, apoptosis and splicing regulation, notably by mediating phosphorylation of CDC25B, phosphorylates BCL2L14, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis, self-renewal of stem cells, thereby contributing to its redirection to the nucleus, Serine/threonine-protein kinase involved in various processes such as cell cycle regulation |
| Immunogen: |
This mouse Melk antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 434-468 amino acids from the C-terminal region of mouse Melk |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Melk antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Melk |
| Short name: |
Melk Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Melk (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16870 |
| Gene: |
MELK |
More about : MELK |
| Long gene name: |
maternal embryonic leucine zipper kinase |
| Synonyms: |
KIAA0175 |
| Locus: |
9p13, 2 |
| Discovery year: |
2003-02-04 |
| GenBank acession: |
D79997 |
| Entrez gene record: |
9833 |
| Pubmed identfication: |
8724849 9136115 |
| RefSeq identity: |
NM_014791 |
| Havana BLAST/BLAT: |
OTTHUMG00000019906 |