| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ACTL6A / BAF53A |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IF |
| Recommended dilutions: |
Immunofluorescence: 1:25, Western blot: 1:500-1:1000 |
| Intented use: |
This BAF53A antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9Z2N8 |
| Purity: |
Antigen affinity |
| Description: |
Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, As neural progenitors exit mitosis and differentiate into neurons, Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors, Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A, DNA replication and probably DNA repair (By similarity), During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state, NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage, Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix, The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth, The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes, This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), and DNA repair, apoptosis, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF), npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, tumor suppressor mediated growth arrest and replicative senescence, which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene (By similarity), BRG1-associated factor 53A is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) |
| Immunogen: |
A portion of amino acids 361-395 from the mouse protein was used as the immunogen for the BAF53A antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the BAF53A antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ACTL6A / BAF53A |
| Short name: |
ACTL6A Antibody / BAF53A |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ACTL6A (Antibody to) / BAF53A |
| Alternative technique: |
antibodies |
| Identity: |
24124 |
| Gene: |
ACTL6A |
More about : ACTL6A |
| Long gene name: |
actin like 6A |
| Synonyms: |
Actl6 BAF53A Arp4 Baf53a INO80K |
| Synonyms name: |
BAF complex 53 kDa subunit BRG1-associated factor actin-related protein 4 INO80 complex subunit K |
| Locus: |
3q26, 33 |
| Discovery year: |
2004-07-12 |
| GenBank acession: |
AK098691 |
| Entrez gene record: |
86 |
| Pubmed identfication: |
9845365 10380635 |
| RefSeq identity: |
NM_004301 |
| Classification: |
INO80 complex SRCAP complex |
| Havana BLAST/BLAT: |
OTTHUMG00000157782 |