| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PHF1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the PHF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This PHF1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O43189 |
| Purity: |
Antigen affinity |
| Description: |
According to another report, According to some reports, Acts by binding to H3K36me3, Involved in DNA damage response and is recruited at double-strand breaks (DSBs), PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980), PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982), Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex, Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex |
| Immunogen: |
This PHF1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the N-terminal region of human PHF1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PHF1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PHF1 |
| Short name: |
PHF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PHF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8919 |
| Gene: |
PHF1 |
More about : PHF1 |
| Long gene name: |
PHD finger protein 1 |
| Synonyms: |
MTF2L2 TDRD19C PCL1 |
| Synonyms name: |
tudor domain containing 19C polycomb-like 1 |
| Locus: |
6p21, 32 |
| Discovery year: |
1997-10-10 |
| GenBank acession: |
AF029678 |
| Entrez gene record: |
5252 |
| Pubmed identfication: |
9545646 18385154 |
| Classification: |
PHD finger proteins Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000031105 |