| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Pou5f1 OCT4 (Zebrafish) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Zebrafish |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Pou5f1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q90270 |
| Purity: |
Antigen affinity |
| Description: |
In contrast to POU2, May play an important role in establishing and specifying rhombomeric segments, Overexpression of POU2 does not have any effect on development, Seems to be required to maintain the cells in a highly undifferentiated state, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure, especially in the process of gastrulation, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation, Involved in early development of embryos |
| Immunogen: |
This Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of zebrafish Pou5f1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Pou5f1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Pou5f1 OCT4 (Zebrafish) |
| Short name: |
Pou5f1 Antibody OCT4 (Zebrafish) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Species: |
Zebrafishes, Zebrafish |
| Alternative name: |
Pou5f1 (Antibody to) OCT4 (Zebrafish) |
| Alternative technique: |
antibodies |
| Identity: |
9221 |
| Gene: |
POU5F1 |
More about : POU5F1 |
| Long gene name: |
POU class 5 homeobox 1 |
| Synonyms gene: |
OTF3 |
| Synonyms gene name: |
POU domain class 5, transcription factor 1 |
| Synonyms: |
OCT3 Oct4 MGC22487 |
| Locus: |
6p21, 33 |
| Discovery year: |
1992-11-05 |
| GenBank acession: |
Z11898 |
| Entrez gene record: |
5460 |
| Pubmed identfication: |
1408763 |
| RefSeq identity: |
NM_002701 |
| Classification: |
POU class homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000031206 |