| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Oct4 (POU5F1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, WB |
| Recommended dilutions: |
Immunofluorescence: 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Oct4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q01860 |
| Purity: |
Purified |
| Description: |
Oct3 is present in mouse oocytes before and after fertilization, Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined, Two forms of POU5F1/OCT4/OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B, When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage, Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development |
| Immunogen: |
A portion of amino acids 1-30 from the human protein was used as the immunogen for this Oct4 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Oct4 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Oct4 (POU5F1) |
| Short name: |
Oct4 Antibody (POU5F1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Oct4 (Antibody to) (POU5F1) |
| Alternative technique: |
antibodies |
| Identity: |
9221 |
| Gene: |
POU5F1 |
More about : POU5F1 |
| Long gene name: |
POU class 5 homeobox 1 |
| Synonyms gene: |
OTF3 |
| Synonyms gene name: |
POU domain class 5, transcription factor 1 |
| Synonyms: |
OCT3 Oct4 MGC22487 |
| Locus: |
6p21, 33 |
| Discovery year: |
1992-11-05 |
| GenBank acession: |
Z11898 |
| Entrez gene record: |
5460 |
| Pubmed identfication: |
1408763 |
| RefSeq identity: |
NM_002701 |
| Classification: |
POU class homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000031206 |