| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
IkB alpha |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the IkB alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This IkB alpha antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P25963 |
| Purity: |
Antigen affinity |
| Description: |
Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, IKBKB) marks them for destruction via the ubiquitination pathway, Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, R is an A or G purine, RELA, The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), and Y is a C or T pyrimidine), or RELB to form the NFKB complex, or T, thereby allowing activation of the NF-kappa-B complex, which inactivate NF-kappa-B by trapping it in the cytoplasm, NFKB1 or NFKB2 is bound to REL |
| Immunogen: |
A portion of amino acids 12-41 from the human protein was used as the immunogen for this IkB alpha antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the IkB alpha antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
IkB alpha |
| Short name: |
IkB alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
IkB a (Antibody to) |
| Alternative technique: |
antibodies |