| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Apoptosis Signal-regulating Kinase 1 (ASK1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the ASK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This ASK1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q99683 |
| Purity: |
Antigen affinity |
| Description: |
ASK1/MAPKKK5/MAP3K5 contains 1, Drosophila, MAP3K5 does not activate MAPK/ERK, MAPK kinase (MKK or MEK), MAPK kinase/MEK, MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPKK4) in vitro, Northern blot analysis shows that transcript is abundantly expressed in human heart and pancreas, The MAP3K5 protein phosphorylates and activates MKK4 (aliases SERK1, The kinases of these signaling cascades are highly conserved, and MAPK kinase kinase (MAPKKK or MEKK), and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, and homologs exist in yeast, and mammalian cells, which in turn activates MAPK, 374 amino acids with all 11 kinase subdomains, Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK) |
| Immunogen: |
A portion of amino acids 944-973 from the human protein was used as the immunogen for this ASK1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ASK1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Signal-regulating Kinase 1 (ASK1) |
| Short name: |
Apoptosis Signal-regulating Kinase 1 Antibody (ASK1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
programmed cellular destruction Signal-regulating phosphorylation catalyst 1 (Antibody to) (ASK1) |
| Alternative technique: |
antibodies |
| Identity: |
6857 |
| Gene: |
MAP3K5 |
More about : MAP3K5 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 5 |
| Synonyms gene: |
MEKK5 |
| Synonyms: |
MAPKKK5 ASK1 |
| Synonyms name: |
apoptosis signal regulating kinase 1 |
| Locus: |
6q23, 3 |
| Discovery year: |
1997-11-14 |
| GenBank acession: |
U67156 |
| Entrez gene record: |
4217 |
| Pubmed identfication: |
9465908 |
| Classification: |
Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000015647 |
| Locus Specific Databases: |
The Globin Gene Server |