| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
BRAF |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB, FACS |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This B-RAF antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P15056 |
| Purity: |
Purified |
| Description: |
Drugs that treat cancers driven by BRAF mutations have been developed, The protein is involved in sending signals inside cells, Two of these drugs, Vemurafenib and Dabrafenib are approved by FDA for treatment of late-stage melanoma, [Wiki], which are involved in directing cell growth, with the protein more formally known as serine/threonine-protein kinase B-Raf, BRAF is a human gene |
| Immunogen: |
A portion of amino acids 424-453 from the human protein was used as the immunogen for this B-RAF antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot the B-RAF antibody and store frozen at -20 deg, Store at 4oC for up to one month |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
BRAF |
| Short name: |
BRAF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
v-raf murine sarcoma viral oncogene homolog B1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
B-RAF1 and BRAF1 and NS7 and RAFB1, BRAF and IDBG-44351 and ENSG00000157764 and 673, BRAF and IDBG-636391 and ENSBTAG00000021761 and, Braf and IDBG-138657 and ENSMUSG00000002413 and 109880, alpha-beta T cell differentiation and biological process this GO :0043368 and positive T cell selection and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0044297 and cell body and cellular component this GO :0046632 and alpha-beta T cell differentiation and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051591 and response to cAMP and biological process this GO :0060291 and long-term synaptic potentiation and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070849 and response to epidermal growth factor and biological process this GO :0071277 and cellular response to calcium ion and biological process this GO :1900026 and positive regulation of substrate adhesion-dependent cell spreading and biological process this GO :2000301 and negative regulation of synaptic vesicle exocytosis and biological process this GO :2000352 and negative regulation of endothelial cell apoptotic process and biological process, nuclei, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004709 and MAP kinase kinase kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005057 and receptor signaling protein activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007268 and synaptic transmission and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010764 and negative regulation of fibroblast migration and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004709 : MAP kinase kinase kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005057 : receptor signaling protein activity and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004709 : MAP kinase kinase kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005057 : receptor signaling protein activity, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0031434 : mitogen-activated protein kinase kinase binding, this GO :0042802 : identical protein binding, this GO :0046982 : protein heterodimerization activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031434 : mitogen-activated protein kinase kinase binding and also this GO :0042802 : identical protein binding and also this GO :0046982 : protein heterodimerization activity, transferring phosphorus-containing groups and molecular function this GO :0030154 and cell differentiation and biological process this GO :0031434 and mitogen-activated protein kinase kinase binding and molecular function this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035690 and cellular response to drug and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043367 and CD4-positive, v-raf murine sarcoma viral oncogene homolog B1 |
| Identity: |
1097 |
| Gene: |
BRAF |
More about : BRAF |
| Long gene name: |
B-Raf proto-oncogene, serine/threonine kinase |
| Synonyms gene name: |
v-raf murine sarcoma viral oncogene homolog B |
| Synonyms: |
BRAF1 |
| Locus: |
7q34 |
| Discovery year: |
1991-07-16 |
| GenBank acession: |
M95712 |
| Entrez gene record: |
673 |
| Pubmed identfication: |
2284096 1565476 |
| RefSeq identity: |
NM_004333 |
| Classification: |
RAF family Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000157457 |
| Locus Specific Databases: |
LRG_299 |