| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ACK1 / Activated CDC42 kinase 1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:500-1000 |
| Intented use: |
This ACK1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q07912 |
| Purity: |
Purified |
| Description: |
The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway, This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain, Activated CDC42 kinase 1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs |
| Immunogen: |
A portion of amino acids 934-964 from the human protein was used as the immunogen for this ACK1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ACK1 antibody and store frozen at -20 deg |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ACK1 / Activated CDC42 kinase 1 |
| Short name: |
ACK1 Antibody / Activated CDC42 kinase 1 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
25kDa) phosphorylation catalyst 1, ACK1 (Antibody to) / Activated cellular division cycle 42 (GTP binding protein |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
25kDa), BT, CDC42 and IDBG-93614 and ENSG00000070831 and 998, CDC42Hs and G25K, Cdc42 and IDBG-198471 and ENSMUSG00000006699 and 12540, Cell surfaces, identical protein binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002040 and sprouting angiogenesis and biological process this GO :0003161 and cardiac conduction system development and biological process this GO :0003334 and keratinocyte development and biological process this GO :0003924 and GTPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005815 and microtubule organizing center and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006184 and GTP catabolic process and biological process this GO :0007015 and actin filament organization and biological process this GO :0007030 and this GO lgi organization and biological process this GO :0007088 and regulation of mitosis and biological process this GO :0007097 and nuclear migration and biological process this GO :0007163 and establishment or maintenance of cell polarity and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0007596 and blood coagulation and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0015031 and protein transport and biological process this GO :0016020 and membrane and cellular component this GO :0019901 and protein kinase binding and molecular function this GO :0021762 and substantia nigra development and biological process this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030141 and secretory granule and cellular component this GO :0030175 and filopodium and cellular component this GO :0030225 and macrophage differentiation and biological process this GO :0030496 and midbody and cellular component this GO :0030742 and GTP-dependent protein binding and molecular function this GO :0031069 and hair follicle morphogenesis and biological process this GO :0031256 and leading edge membrane and cellular component this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031295 and T cell costimulation and biological process this GO :0031333 and negative regulation of protein complex assembly and biological process this GO :0031424 and keratinization and biological process this GO :0031435 and mitogen-activated protein kinase kinase kinase binding and molecular function this GO :0031647 and regulation of protein stability and biological process this GO :0031996 and thioesterase binding and molecular function this GO :0032467 and positive regulation of cytokinesis and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0034191 and apolipoprotein A-I receptor binding and molecular function this GO :0034332 and adherens junction organization and biological process this GO :0034613 and cellular protein localization and biological process this GO :0035088 and establishment or maintenance of apical/basal cell polarity and biological process this GO :0035264 and multicellular organism growth and biological process this GO :0036336 and dendritic cell migration and biological process this GO :0036464 and cytoplasmic ribonucleoprotein granule and cellular component this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0042059 and negative regulation of epidermal growth factor receptor signaling pathway and biological process this GO :0042176 and regulation of protein catabolic process and biological process this GO :0042692 and muscle cell differentiation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042995 and cell projection and cellular component this GO :0043005 and neuron projection and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043497 and regulation of protein heterodimerization activity and biological process this GO :0043525 and positive regulation of neuron apoptotic process and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045177 and apical part of cell and cellular component this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045859 and regulation of protein kinase activity and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046847 and filopodium assembly and biological process this GO :0048554 and positive regulation of metalloenzyme activity and biological process this GO :0048664 and neuron fate determination and biological process this GO :0048730 and epidermis morphogenesis and biological process this GO :0051017 and actin filament bundle assembly and biological process this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :0051149 and positive regulation of muscle cell differentiation and biological process this GO :0051233 and spindle midzone and cellular component this GO :0051246 and regulation of protein metabolic process and biological process this GO :0051489 and regulation of filopodium assembly and biological process this GO :0051647 and nucleus localization and biological process this GO :0051683 and establishment of this GO lgi localization and biological process this GO :0051835 and positive regulation of synapse structural plasticity and biological process this GO :0051988 and regulation of attachment of spindle microtubules to kinetochore and biological process this GO :0060047 and heart contraction and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060661 and submandibular salivary gland formation and biological process this GO :0060684 and epithelial-mesenchymal cell signaling and biological process this GO :0060789 and hair follicle placode formation and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071338 and positive regulation of hair follicle cell proliferation and biological process this GO :0071944 and cell periphery and cellular component this GO :0072384 and organelle transport along microtubule and biological process this GO :0072686 and mitotic spindle and cellular component this GO :0090135 and actin filament branching and biological process this GO :0090136 and epithelial cell-cell adhesion and biological process this GO :0090316 and positive regulation of intracellular protein transport and biological process this GO :1900026 and positive regulation of substrate adhesion-dependent cell spreading and biological process, this GO :0003924 : GTPase activity, this GO :0003924 : GTPase activity and also this GO :0005515 : protein binding and also this GO :0005525 : GTP binding and also this GO :0019901 : protein kinase binding and also this GO :0030742 : GTP-dependent protein binding and also this GO :0031435 : mitogen-activated protein kinase kinase kinase binding and also this GO :0031996 : thioesterase binding and also this GO :0034191 : apolipoprotein A-I receptor binding and also this GO :0042802 : identical protein binding, this GO :0005515 : protein binding, this GO :0005525 : GTP binding, this GO :0019901 : protein kinase binding, this GO :0030742 : GTP-dependent protein binding, this GO :0031435 : mitogen-activated protein kinase kinase kinase binding, this GO :0031996 : thioesterase binding, this GO :0034191 : apolipoprotein A-I receptor binding, this GO :0042802 : identical protein binding, 56113 and IDBG-645532 and ENSBTAG00000001700 and 532712, cell division cycle 42 (GTP binding protein |
| Identity: |
1736 |
| Gene: |
CDC42 |
More about : CDC42 |
| Long gene name: |
cell division cycle 42 |
| Synonyms gene name: |
25kD) cell division cycle 42 (GTP binding protein, 25kDa) , cell division cycle 42 (GTP-binding protein |
| Synonyms: |
G25K CDC42Hs |
| Synonyms name: |
25kDa , GTP binding protein |
| Locus: |
1p36, 12 |
| Discovery year: |
1991-06-06 |
| GenBank acession: |
BC018266 |
| Entrez gene record: |
998 |
| Pubmed identfication: |
2124704 2122236 |
| RefSeq identity: |
NM_001791 |
| Classification: |
Rho family GTPases |
| Havana BLAST/BLAT: |
OTTHUMG00000002753 |