| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
AMPK alpha 2 (PRKAA2) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the AMPK alpha 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This AMPK alpha 2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P54646 |
| Purity: |
Purified |
| Description: |
AMPK is a heterotrimer consisting of an alpha catalytic subunit, AMPK is activated, AMPK is an important energy-sensing enzyme that monitors cellular energy status, In response to cellular metabolic stresses, Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia, and non-catalytic beta and gamma subunits, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol, The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK) |
| Immunogen: |
A portion of amino acids 453-483 from the human protein was used as the immunogen for this AMPK alpha 2 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the AMPK alpha 2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
AMPK alpha 2 (PRKAA2) |
| Short name: |
AMPK alpha 2 Antibody (PRKAA2) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
AMP-activated, a 2 catalytic subunit), AMPK a 2 (Antibody to) (protein phosphorylation catalyst |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AMP-activated, AMPK and AMPK2 and AMPKa2 and PRKAA, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006633 and fatty acid biosynthetic process and biological process this GO :0006695 and cholesterol biosynthetic process and biological process this GO :0006853 and carnitine shuttle and biological process this GO :0006914 and autophagy and biological process this GO :0006950 and response to stress and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007165 and signal transduction and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008610 and lipid biosynthetic process and biological process this GO :0010508 and positive regulation of autophagy and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0016772 and transferase activity, PRKAA2 and IDBG-638934 and ENSBTAG00000018747 and 538954, PRKAA2 and IDBG-99136 and ENSG00000162409 and 5563, Prkaa2 and IDBG-169837 and ENSMUSG00000028518 and 108079, alpha 2 catalytic subunit, nuclei, this GO :0003682 and chromatin binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004679 and AMP-activated protein kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006112 and energy reserve metabolic process and biological process this GO :0006351 and transcription, this GO :0003682 : chromatin binding, this GO :0003682 : chromatin binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004679 : AMP-activated protein kinase activity and also this GO :0004712 : protein serine/threonine/tyrosine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004679 : AMP-activated protein kinase activity, this GO :0004712 : protein serine/threonine/tyrosine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0035174 : histone serine kinase activity, this GO :0046872 : metal ion binding, this GO :0047322 : [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity, this GO :0050405 : [acetyl-CoA carboxylase] kinase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0035174 : histone serine kinase activity and also this GO :0046872 : metal ion binding and also this GO :0047322 : [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity and also this GO :0050405 : [acetyl-CoA carboxylase] kinase activity, transferring phosphorus-containing groups and molecular function this GO :0031669 and cellular response to nutrient levels and biological process this GO :0032007 and negative regulation of TOR signaling and biological process this GO :0035174 and histone serine kinase activity and molecular function this GO :0035404 and histone-serine phosphorylation and biological process this GO :0042149 and cellular response to glucose starvation and biological process this GO :0042304 and regulation of fatty acid biosynthetic process and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042752 and regulation of circadian rhythm and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044255 and cellular lipid metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045821 and positive regulation of glycolytic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0047322 and [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity and molecular function this GO :0048511 and rhythmic process and biological process this GO :0050405 and [acetyl-CoA carboxylase] kinase activity and molecular function this GO :0055089 and fatty acid homeostasis and biological process this GO :0061024 and membrane organization and biological process this GO :2000505 and regulation of energy homeostasis and biological process, protein kinase |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Synonyms: |
AMPK AMPKa2 |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |