| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
DHFR |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This DHFR antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P00375 |
| Purity: |
Purified |
| Description: |
Dihydrofolate reductase deficiency has been linked to megaloblastic anemia, While the functional dihydrofolate reductase gene has been mapped to chromosome 5, a methyl group shuttle required for the de novo synthesis of purines, and certain amino acids, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes, thymidylic acid, Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate |
| Immunogen: |
A portion of amino acids 135-164 from mouse Dihydrofolate reductase was used as the immunogen for this DHFR antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the DHFR antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
DHFR |
| Short name: |
DHFR Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
DHFR (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2861 |
| Gene: |
DHFR |
More about : DHFR |
| Long gene name: |
dihydrofolate reductase |
| Locus: |
5q14, 1 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
1719 |
| RefSeq identity: |
NM_000791 |
| Havana BLAST/BLAT: |
OTTHUMG00000162529 |