| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
YAP1 (phospho-S127) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-YAP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P46937 |
| Purity: |
Antigen affinity |
| Description: |
Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3), Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, Plays a key role to control cell proliferation in response to cell contact, The core of this pathway is composed of a kinase cascade wherein MST1/MST2, The presence of TEAD transcription factors are required for it to stimulate gene expression, anchorage-independent growth, and cell migration, and epithelial mesenchymal transition (EMT) induction, cell death, cell growth, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ, Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis |
| Immunogen: |
This phospho-YAP antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS127 of human YAP1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-YAP antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
YAP1 (phospho-S127) |
| Short name: |
YAP1 Antibody (phospho-S127) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
YAP1 (Antibody to) (phosphorilated-S127) |
| Alternative technique: |
antibodies |
| Identity: |
16262 |
| Gene: |
YAP1 |
More about : YAP1 |
| Long gene name: |
Yes associated protein 1 |
| Synonyms gene name: |
65kDa , Yes-associated protein 1 |
| Synonyms: |
YAP65 |
| Locus: |
11q22, 1 |
| Discovery year: |
2001-07-17 |
| Entrez gene record: |
10413 |
| Pubmed identfication: |
7782338 |
| RefSeq identity: |
NM_006106 |
| Havana BLAST/BLAT: |
OTTHUMG00000167322 |