| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-IkBa (pSer32)) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-IkBa antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-IkBa antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P25963 |
| Purity: |
Antigen affinity |
| Description: |
Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime, MIM 600664, MIM 603258) marks them for destruction via the ubiquitination pathway, MIM 604495), Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, RELA (MIM 164014), The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, or IKBKB, or RELB (MIM 604758) to form the NFKB complex, thereby allowing activation of the NF-kappa-B complex, which inactivate NF-kappa-B by trapping it in the cytoplasm, NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910) |
| Immunogen: |
This phospho-IkBa antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS32 of human NFKBIA |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-IkBa antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-IkBa (pSer32) |
| Short name: |
Phospho-IkBa Antibody (pSer32)) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-IkBa (Antibody to) (pSer32)) |
| Alternative technique: |
antibodies |