| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-HER3 (pY1289) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
DB, ELISA, WB |
| Recommended dilutions: |
Dot blot: 1:500, Western blot: 1:1000 |
| Notes: |
Titration of the phospho-HER3 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-HER3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P21860 |
| Purity: |
Antigen affinity |
| Description: |
Alternate transcriptional splice variants encoding different isoforms have been characterized, Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation, However, It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation, One isoform lacks the intermembrane region and is secreted outside the cell, This form acts to modulate the activity of the membrane-bound form, This membrane-bound protein has a neuregulin binding domain but not an active kinase domain, and breast tumors, bladder, including prostate, it does form heterodimers with other EGF receptor family members which do have kinase activity, This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases |
| Immunogen: |
This phospho-HER3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1289 of human HER3/ERBB3 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-HER3 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-HER3 (pY1289) |
| Short name: |
Phospho-HER3 Antibody (pY1289) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-HER3 (Antibody to) (pY1289) |
| Alternative technique: |
antibodies |
| Identity: |
3431 |
| Gene: |
ERBB3 |
More about : ERBB3 |
| Long gene name: |
erb-b2 receptor tyrosine kinase 3 |
| Synonyms gene: |
LCCS2 |
| Synonyms gene name: |
lethal congenital contracture syndrome 2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
| Synonyms: |
HER3 |
| Synonyms name: |
human epidermal growth factor receptor 3 |
| Locus: |
12q13, 2 |
| Discovery year: |
1990-07-15 |
| GenBank acession: |
M34309 |
| Entrez gene record: |
2065 |
| RefSeq identity: |
NM_001982 |
| Classification: |
Erb-b2 receptor tyrosine kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000170140 |
| Locus Specific Databases: |
LRG_996 |