| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
p-NANOG (pS71) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
DB, ELISA, IF |
| Recommended dilutions: |
Dot blot: 1:500, Immunofluorescence: 1:10-1:50 |
| Notes: |
Titration of the p-NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This p-NANOG antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9H9S0 |
| Purity: |
Antigen affinity |
| Description: |
It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3', It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, NANOG acts as a transcriptional activator or repressor, This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes, When overexpressed, this protein promotes cells to enter into S phase and proliferation, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal |
| Immunogen: |
This p-NANOG antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS71 of human Nanog |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the p-NANOG antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
p-NANOG (pS71) |
| Short name: |
p-NANOG Antibody (pS71) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
p-NANOG (Antibody to) (pS71) |
| Alternative technique: |
antibodies |
| Identity: |
20857 |
| Gene: |
NANOG |
More about : NANOG |
| Long gene name: |
Nanog homeobox |
| Synonyms: |
FLJ12581 FLJ40451 |
| Locus: |
12p13, 31 |
| Discovery year: |
2003-09-10 |
| GenBank acession: |
AB093576 |
| Entrez gene record: |
79923 |
| Pubmed identfication: |
12787505 12787504 |
| RefSeq identity: |
NM_024865 |
| Classification: |
NKL subclass homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000166019 |