| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-MDM2 (pS395) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-MDM2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q00987 |
| Purity: |
Antigen affinity |
| Description: |
MDM2 also affects the cell cycle, Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, This protein has E3 ubiquitin ligase activity, This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, and tumorigenesis through interactions with other proteins, as part of an autoregulatory negative feedback loop, diminishing its tumor suppressor function, including retinoblastoma 1 and ribosomal protein L5, which targets tumor protein p53 for proteasomal degradation, MDM2 is a target of the transcription factor tumor protein p53, apoptosis |
| Immunogen: |
This phospho-MDM2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS395 of human MDM2 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-MDM2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-MDM2 (pS395) |
| Short name: |
Phospho-MDM2 Antibody (pS395) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-MDM2 (Antibody to) (pS395) |
| Alternative technique: |
antibodies |
| Identity: |
6973 |
| Gene: |
MDM2 |
More about : MDM2 |
| Long gene name: |
MDM2 proto-oncogene |
| Synonyms gene name: |
E3 ubiquitin protein ligase , human homolog of, mouse double minute 2, p53 binding protein (mouse) Mdm2 p53 binding protein homolog (mouse) MDM2 proto-oncogene, p53-binding protein Mdm2, transformed 3T3 cell double minute 2 |
| Synonyms: |
HDM2 MGC5370 |
| Locus: |
12q15 |
| Discovery year: |
1993-12-10 |
| Entrez gene record: |
4193 |
| Pubmed identfication: |
1614537 16905769 |
| RefSeq identity: |
NM_006880 |
| Classification: |
Zinc fingers RANBP2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000142827 |