| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LC3B (phospho-T12) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-LC3B antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9GZQ8 |
| Purity: |
Antigen affinity |
| Description: |
LC3-I, LC3-II, MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits, MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B, Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, These proteins are involved in formation of autophagosomal vacuoles (autophagosomes), This is activated by APG7L/ATG7, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole), MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton |
| Immunogen: |
This phospho-LC3B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT12 of human LC3B |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-LC3B antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LC3B (phospho-T12) |
| Short name: |
LC3B Antibody (phospho-T12) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LC3B (Antibody to) (phosphorilated-T12) |
| Alternative technique: |
antibodies |