| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-Dnmt1 (pS714) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-Dnmt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-Dnmt1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P26358 |
| Purity: |
Antigen affinity |
| Description: |
3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality, Dnmt2 and Dnmt3, Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation, Dnmt3a and Dnmt3b, E2F1, Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer, The Dnmt3 family members, To date, and HDAC1, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells, genomic imprinting and is essential for mammalian development, histone deacetylase and sequence-specific DNA binding activity, Methylation of DNA at cytosine residues plays an important role in regulation of gene expression |
| Immunogen: |
This phospho-Dnmt1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS714 of human Dnmt1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-Dnmt1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-Dnmt1 (pS714) |
| Short name: |
Phospho-Dnmt1 Antibody (pS714) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-Dnmt1 (Antibody to) (pS714) |
| Alternative technique: |
antibodies |