| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
EIF4E (phospho-S209) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-EIF4E antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P06730 |
| Purity: |
Antigen affinity |
| Description: |
EIF4E and EIF4G1/EIF4G3, EIF4E is also known to interact with other partners, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, It is composed of at least EIF4A, Nonphosphorylated EIF4EBP1, Rapamycin can attenuate insulin stimulation, The interaction with EIF4ENIF1 mediates the import into the nucleus, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation, mediated by FKBPs, the composition of which varies with external and internal environmental conditions, eIF4F is a multi-subunit complex |
| Immunogen: |
This phospho-EIF4E antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS209 of human EIF4E |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-EIF4E antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
EIF4E (phospho-S209) |
| Short name: |
EIF4E Antibody (phospho-S209) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
EIF4E (Antibody to) (phosphorilated-S209) |
| Alternative technique: |
antibodies |
| Identity: |
3287 |
| Gene: |
EIF4E |
More about : EIF4E |
| Long gene name: |
eukaryotic translation initiation factor 4E |
| Synonyms gene: |
EIF4EL1 EIF4F |
| Synonyms: |
EIF4E1 |
| Locus: |
4q23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
M15353 |
| Entrez gene record: |
1977 |
| Pubmed identfication: |
9330633 1916814 |
| RefSeq identity: |
NM_001968 |
| Havana BLAST/BLAT: |
OTTHUMG00000161090 |