| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-SMAD3 (pS213) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the phospho-SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-SMAD3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P84022 |
| Purity: |
Antigen affinity |
| Description: |
Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif, Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response, SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes, The phosphorylated R-SMAD translocate into nucleus, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase, where they regulate transcription of target genes, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma, SMAD3 |
| Immunogen: |
This phospho-SMAD3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS213 of human SMAD3 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-SMAD3 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-SMAD3 (pS213) |
| Short name: |
Phospho-SMAD3 Antibody (pS213) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-SMAD family member 3 (Antibody to) (pS213) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006810 and transport and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006955 and immune response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007369 and gastrulation and biological process this GO :0007492 and endoderm development and biological process this GO :0008013 and beta-catenin binding and molecular function this GO :0008134 and transcription factor binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009880 and embryonic pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010694 and positive regulation of alkaline phosphatase activity and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0016202 and regulation of striated muscle tissue development and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0019049 and evasion or tolerance of host defenses by virus and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0019902 and phosphatase binding and molecular function this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030501 and positive regulation of bone mineralization and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030618 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045930 and negative regulation of mitotic cell cycle and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046332 and SMAD binding and molecular function this GO :0048340 and paraxial mesoderm morphogenesis and biological process this GO :0048589 and developmental growth and biological process this GO :0048617 and embryonic foregut morphogenesis and biological process this GO :0048701 and embryonic cranial skeleton morphogenesis and biological process this GO :0050678 and regulation of epithelial cell proliferation and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050821 and protein stabilization and biological process this GO :0050927 and positive regulation of positive chemotaxis and biological process this GO :0051098 and regulation of binding and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051894 and positive regulation of focal adhesion assembly and biological process this GO :0060039 and pericardium development and biological process this GO :0060290 and transdifferentiation and biological process this GO :0061045 and negative regulation of wound healing and biological process this GO :0070306 and lens fiber cell differentiation and biological process this GO :0070410 and co-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0071141 and SMAD protein complex and cellular component this GO :0071144 and SMAD2-SMAD3 protein complex and cellular component this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097296 and activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway and biological process this GO :1901313 and positive regulation of gene expression involved in extracellular matrix organization and biological process, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, nuclei, pathway-specific cytoplasmic mediator activity, pathway-specific cytoplasmic mediator activity and also this GO :0031490 : chromatin DNA binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0035326 : enhancer binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043130 : ubiquitin binding and also this GO :0043425 : bHLH transcription factor binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046332 : SMAD binding and also this GO :0070410 : co-SMAD binding and also this GO :0070412 : R-SMAD binding, pathway-specific cytoplasmic mediator activity and molecular function this GO :0030878 and thyroid gland development and biological process this GO :0031053 and primary miRNA processing and biological process this GO :0031490 and chromatin DNA binding and molecular function this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032332 and positive regulation of chondrocyte differentiation and biological process this GO :0032731 and positive regulation of interleukin-1 beta production and biological process this GO :0032909 and regulation of transforming growth factor beta2 production and biological process this GO :0032916 and positive regulation of transforming growth factor beta3 production and biological process this GO :0032924 and activin receptor signaling pathway and biological process this GO :0033689 and negative regulation of osteoblast proliferation and biological process this GO :0035326 and enhancer binding and molecular function this GO :0035413 and positive regulation of catenin import into nucleus and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038092 and nodal signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0042110 and T cell activation and biological process this GO :0042177 and negative regulation of protein catabolic process and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042993 and positive regulation of transcription factor import into nucleus and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043130 and ubiquitin binding and molecular function this GO :0043234 and protein complex and cellular component this GO :0043235 and receptor complex and cellular component this GO :0043425 and bHLH transcription factor binding and molecular function this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045216 and cell-cell junction organization and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045893 and positive regulation of transcription, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000988 and protein binding transcription factor activity and molecular function this GO :0001102 and RNA polymerase II activating transcription factor binding and molecular function this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001657 and ureteric bud development and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001707 and mesoderm formation and biological process this GO :0001756 and somitogenesis and biological process this GO :0001889 and liver development and biological process this GO :0001933 and negative regulation of protein phosphorylation and biological process this GO :0001947 and heart looping and biological process this GO :0002076 and osteoblast development and biological process this GO :0002520 and immune system development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005160 and transforming growth factor beta receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005637 and nuclear inner membrane and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0000988 : protein binding transcription factor activity and also this GO :0001102 : RNA polymerase II activating transcription factor binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005160 : transforming growth factor beta receptor binding and also this GO :0005515 : protein binding and also this GO :0005518 : collagen binding and also this GO :0008013 : beta-catenin binding and also this GO :0008134 : transcription factor binding and also this GO :0008270 : zinc ion binding and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0019902 : phosphatase binding and also this GO :0030618 : transforming growth factor beta receptor, this GO :0000988 : protein binding transcription factor activity, this GO :0001102 : RNA polymerase II activating transcription factor binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003690 : double-stranded DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005160 : transforming growth factor beta receptor binding, this GO :0005515 : protein binding, this GO :0005518 : collagen binding, this GO :0008013 : beta-catenin binding, this GO :0008134 : transcription factor binding, this GO :0008270 : zinc ion binding, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0019902 : phosphatase binding, this GO :0030618 : transforming growth factor beta receptor, this GO :0031490 : chromatin DNA binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0035326 : enhancer binding, this GO :0042803 : protein homodimerization activity, this GO :0043130 : ubiquitin binding, this GO :0043425 : bHLH transcription factor binding, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046332 : SMAD binding, this GO :0070410 : co-SMAD binding, this GO :0070412 : R-SMAD binding, transforming growth factor beta receptor, SMAD family member 3 |
| Identity: |
6769 |
| Gene: |
SMAD3 |
More about : SMAD3 |
| Long gene name: |
SMAD family member 3 |
| Synonyms gene: |
MADH3 |
| Synonyms gene name: |
MAD, mothers against DPP homolog 3 (Drosophila) , mothers against decapentaplegic homolog 3 (Drosophila) SMAD |
| Synonyms: |
JV15-2 HsT17436 |
| Locus: |
15q22, 33 |
| Discovery year: |
1996-11-15 |
| GenBank acession: |
BC050743 |
| Entrez gene record: |
4088 |
| Pubmed identfication: |
8774881 8673135 |
| RefSeq identity: |
NM_005902 |
| Classification: |
SMAD family |
| Havana BLAST/BLAT: |
OTTHUMG00000133230 |
| Locus Specific Databases: |
The Globin Gene Server |