| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-p27Kip1 (pT157) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, DB |
| Recommended dilutions: |
Dot blot: 1:500, IHC (Paraffin): 1:50-1:100 |
| Notes: |
Titration of the phospho-p27Kip1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-p27Kip1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P46527 |
| Purity: |
Antigen affinity |
| Description: |
The degradation of this protein, The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1, is required for the cellular transition from quiescence to the proliferative state, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, which shares a limited similarity with CDK inhibitor CDKN1A/p21, p27Kip1 is a cyclin-dependent kinase inhibitor |
| Immunogen: |
This phospho-p27Kip1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT157 of human p27Kip1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-p27Kip1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-p27Kip1 (pT157) |
| Short name: |
Phospho-p27Kip1 Antibody (pT157) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-p27Kip1 (Antibody to) (pT157) |
| Alternative technique: |
antibodies |