| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-p65 (pS536) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the phospho-p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-p65 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q04206 |
| Purity: |
Antigen affinity |
| Description: |
Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, [supplied by OMIM], thereby allowing activation of the NFKB complex, which inactivates NFKB by trapping it in the cytoplasm, The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB) |
| Immunogen: |
This phospho-p65 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS536 of human NFKBp65 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-p65 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-p65 (pS536) |
| Short name: |
Phospho-p65 Antibody (pS536) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-p65 (Antibody to) (pS536) |
| Alternative technique: |
antibodies |