| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CDC25B (phospho-S187) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the phospho-CDC25B antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-CDC25B antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P30305 |
| Purity: |
Antigen affinity |
| Description: |
CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis, CDC25B has oncogenic properties, CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals, Multiple transcript variants for this gene exist, The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2, although its role in tumor formation has not been determined, CDC25B is a member of the CDC25 family of phosphatases |
| Immunogen: |
This phospho-CDC25B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS187 of human CDC25B |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-CDC25B antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CDC25B (phospho-S187) |
| Short name: |
CDC25B Antibody (phospho-S187) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CDC25B (Antibody to) (phosphorilated-S187) |
| Alternative technique: |
antibodies |
| Identity: |
1726 |
| Gene: |
CDC25B |
More about : CDC25B |
| Long gene name: |
cell division cycle 25B |
| Synonyms gene name: |
cell division cycle 25B cell division cycle 25 homolog B (S, cerevisiae) cell division cycle 25 homolog B (S, pombe) |
| Locus: |
20p13 |
| Discovery year: |
1992-07-31 |
| Entrez gene record: |
994 |
| Pubmed identfication: |
1836978 |
| RefSeq identity: |
NM_021874 |
| Classification: |
Class III Cys-based CDC25 phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000031764 |