| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-Bad (pS118) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the phospho-Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-Bad antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q92934 |
| Purity: |
Antigen affinity |
| Description: |
BCL-2 family members are known to be regulators of programmed cell death, Bad is phosphorylated on one or more of Ser-75, Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins, Proapoptotic activity of this protein is regulated through its phosphorylation, Protein kinases AKT and MAP kinase, Ser-118 and Ser-134 in response to survival stimuli, Ser-118 the major site of protein kinase A (CAPK) phosphorylation, Ser-99, Ser-99 is the major site of AKT/PKB phosphorylation, This interaction then facilitates the phosphorylation at Ser-118, This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, a site within the BH3 motif, and reversing their death repressor activity, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein, leading to the release of Bcl-X(L) and the promotion of cell survival, which blocks its pro-apoptotic activity, Bad is a member of the BCL-2 family |
| Immunogen: |
This phospho-Bad antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human Bad |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-Bad antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-Bad (pS118) |
| Short name: |
Phospho-Bad Antibody (pS118) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-BCL2-associated a this GO nist on cell death (Antibody to) (pS118) |
| Alternative technique: |
antibodies |